Composite

Part:BBa_K4350825:Design

Designed by: Ryan Riopel   Group: iGEM22_UBC-Okanagan   (2022-10-09)


nnCPH Transcriptional Unit


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 938
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Each component of this transcriptional unit required complementary fusion sites for proper golden gate assembly with the appropriate Type IIS restriction endonucleases.


Source

This transcriptional unit was assembled by UBC-Okanagan iGEM using golden gate cloning, acquiring the luciferase CDS from https://doi.org/10.1073/pnas.1803615115, codon-optimizing it for Synechocystis sp. PCC 6803. The promoter and terminator were both acquired from the CyanoGate Kit in level 0 backbones (https://doi.org/10.1073/pnas.1803615115).

References